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Proteome Profiler Human Phospho-MAPK Array Kit

(ARY002B)



概述
货号 ARY002B
简介 Contains 4 membranes - each spotted in duplicate with 26 different MAPK antibodies
别名 MAP Kinase (MAPK) Array Kit
性能
供应商 R&D Systems
试剂盒组分
  • 4-Array Membranes
  • 4-Well Multi-dish
  • Lysis Buffer
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed product datasheet with protocol
Simultaneously detect the relative phosphorylation of these of these kinases in a single sample.
Akt1 HSP27 p38 beta
Akt2 JNK1 p38 delta
Akt3 JNK2 p38 gamma
Akt pan JNK3 p53
CREB JNK pan p70 S6K
ERK1 MKK3 RSK1
ERK2 MKK6 RSK2
GSK-3 alpha/beta MSK2 TOR
GSK-3 beta p38 alpha  
Entrez Gene IDs 5594 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Transmembrane TNF-alpha Reverse Signaling Inhibits Lipopolysaccharide-Induced Proinflammatory Cytokine Formation in Macrophages by Inducing TGF-beta: Therapeutic Implications.
Authors: Pallai A, Kiss B, Vereb G, Armaka M, Kollias G, Szekanecz Z, Szondy Z
J Immunol, 2016;196(3):1146-57.
Species: Human
Sample Type: Cell Lysates

Human and mouse monocytes display distinct signalling and cytokine profiles upon stimulation with FFAR2/FFAR3 short-chain fatty acid receptor agonists
Sci Rep, 2016;6(0):34145.
Species: Human
Sample Type: Cell Culture Supernates

IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy
Authors: Neal L Millar
Sci Rep, 2016;6(0):27149.
Species: Human
Sample Type: Tissue Lysate

Improving Drug Penetrability with iRGD Leverages the Therapeutic Response to Sorafenib and Doxorubicin in Hepatocellular Carcinoma.
Authors: Schmithals C, Koberle V, Korkusuz H, Pleli T, Kakoschky B, Augusto E, Ibrahim A, Arencibia J, Vafaizadeh V, Groner B, Korf H, Kronenberger B, Zeuzem S, Vogl T, Waidmann O, Piiper A
Cancer Res, 2015;75(15):3147-54.
Species: Human
Sample Type: Tissue Homogenates

Elafin drives poor outcome in high-grade serous ovarian cancers and basal-like breast tumors.
Authors: Labidi-Galy S, Clauss A, Ng V, Duraisamy S, Elias K, Piao H, Bilal E, Davidowitz R, Lu Y, Badalian-Very G, Gyorffy B, Kang U, Ficarro S, Ganesan S, Mills G, Marto J, Drapkin R
Oncogene, 2015;34(3):373-83.
Species: Human
Sample Type: Cell Lysates

MicroRNA29a regulates IL-33-mediated tissue remodelling in tendon disease.
Authors: Millar N, Gilchrist D, Akbar M, Reilly J, Kerr S, Campbell A, Murrell G, Liew F, Kurowska-Stolarska M, McInnes I
Nat Commun, 2015;6(0):6774.
Species: Human
Sample Type: Whole Cells

Sortilin mediates the release and transfer of exosomes in concert with two tyrosine kinase receptors.
Authors: Wilson C, Naves T, Vincent F, Melloni B, Bonnaud F, Lalloue F, Jauberteau M
J Cell Sci, 2014;127(0):3983-97.
Species: Human
Sample Type: Cell Lysates

Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets.
Authors: Stefanska B, Cheishvili D, Suderman M, Arakelian A, Huang J, Hallett M, Han Z, Al-Mahtab M, Akbar S, Khan W, Raqib R, Tanvir I, Khan H, Rabbani S, Szyf M
Clin Cancer Res, 2014;20(12):3118-32.
Species: Human
Sample Type: Cell Lysates

Galectin-1 has potential prognostic significance and is implicated in clear cell renal cell carcinoma progression through the HIF/mTOR signaling axis.
Authors: White N, Masui O, Newsted D, Scorilas A, Romaschin A, Bjarnason G, Siu K, Yousef G
Br J Cancer, 2014;110(5):1250-9.
Species: Human
Sample Type: Cell Lysates

The niche component periostin is produced by cancer-associated fibroblasts, supporting growth of gastric cancer through ERK activation.
Authors: Kikuchi Y, Kunita A, Iwata C, Komura D, Nishiyama T, Shimazu K, Takeshita K, Shibahara J, Kii I, Morishita Y, Yashiro M, Hirakawa K, Miyazono K, Kudo A, Fukayama M, Kashima T
Am J Pathol, 2014;184(3):859-70.
Species: Human
Sample Type: Cell Lysate

Molecular mechanisms underlying synergistic adhesion of sickle red blood cells by hypoxia and low nitric oxide bioavailability.
Authors: Gutsaeva, Diana R, Montero-Huerta, Pedro, Parkerson, James B, Yerigenahally, Shobha D, Ikuta, Tohru, Head, C Alvin
Blood, 2014;123(12):1917-26.
Species: Human
Sample Type: Whole Cells

TGFbeta activated kinase 1 (TAK1) at the crossroad of B cell receptor and Toll-like receptor 9 signaling pathways in human B cells.
Authors: Szili D, Banko Z, Toth E, Nagy G, Rojkovich B, Gati T, Simon M, Herincs Z, Sarmay G
PLoS ONE, 2014;9(5):e96381.
Species: Human
Sample Type: Cell Lysate

COX-2-independent effects of celecoxib sensitize lymphoma B cells to TRAIL-mediated apoptosis.
Authors: Gallouet A, Travert M, Bresson-Bepoldin L, Guilloton F, Pangault C, Caulet-Maugendre S, Lamy T, Tarte K, Guillaudeux T
Clin Cancer Res, 2014;20(10):2663-73.
Species: Human
Sample Type: Whole Cells

Lack of Matrilin-2 favors liver tumor development via Erk1/2 and GSK-3beta pathways in vivo.
Authors: Fullar A, Baghy K, Deak F, Peterfia B, Zsak Y, Tatrai P, Schaff Z, Dudas J, Kiss I, Kovalszky I
PLoS ONE, 2014;9(4):e93469.
Species: Mouse
Sample Type: Homogenized Tissue

Extract of Cordyceps militaris inhibits angiogenesis and suppresses tumor growth of human malignant melanoma cells.
Authors: Ruma I, Putranto E, Kondo E, Watanabe R, Saito K, Inoue Y, Yamamoto K, Nakata S, Kaihata M, Murata H, Sakaguchi M
Int J Oncol, 2014;45(1):209-18.
Species: Human
Sample Type: Cell Lysates

Sunlight UV-induced skin cancer relies upon activation of the p38alpha signaling pathway.
Authors: Liu K, Yu D, Cho Y, Bode A, Ma W, Yao K, Li S, Li J, Bowden G, Dong Z, Dong Z
Cancer Res, 2013;73(7):2181-8.
Species: Human
Sample Type: Cell Lysate

Survival of cancer stem cells under hypoxia and serum depletion via decrease in PP2A activity and activation of p38-MAPKAPK2-Hsp27.
Authors: Lin, Shih-Pei, Lee, Yi-Ting, Wang, Jir-You, Miller, Stephani, Chiou, Shih-Hwa, Hung, Mien-Chi, Hung, Shih-Chi
PLoS ONE, 2012;7(11):e49605.
Species: Human
Sample Type: Cell Lysates

Differential function of Akt1 and Akt2 in human adipocytes.
Mol. Cell. Endocrinol., 2012;358(1):135-43.
Species: Human
Sample Type: Cell Lysates

The helicase HAGE expressed by malignant melanoma-initiating cells is required for tumor cell proliferation in vivo.
Authors: Linley AJ, Mathieu MG, Miles AK
J. Biol. Chem., 2012;287(17):13633-43.
Species: Human
Sample Type: Cell Lysates

RUNX3-mediated transcriptional inhibition of Akt suppresses tumorigenesis of human gastric cancer cells.
Authors: Lin F, Liu Y, Lai C, Shan Y, Cheng H, Hsu P, Lee C, Lee Y, Wang H, Wang C, Cheng J, Hsiao M, Lu P
Oncogene, 2012;31(39):4302-16.
Species: Human
Sample Type: Cell Lysates

Cross-suppression of EGFR ligands amphiregulin and epiregulin and de-repression of FGFR3 signalling contribute to cetuximab resistance in wild-type KRAS tumour cells.
Authors: Oliveras-Ferraros C, Cufi S, Queralt B, Vazquez-Martin A, Martin-Castillo B, de Llorens R, Bosch-Barrera J, Brunet J, Menendez JA
Br. J. Cancer, 2012;106(8):1406-14.
Species: Human
Sample Type: Cell Lysates

Lapatinib, a preventive/therapeutic agent against mammary cancer, suppresses RTK-mediated signaling through multiple signaling pathways.
Authors: Li J, Cho YY, Langfald A
Cancer Prev Res (Phila), 2011;4(8):1190-7.
Species: Rat
Sample Type: Tissue Homogenates

Mechanisms underlying gemcitabine resistance in pancreatic cancer and sensitisation by the iMiD lenalidomide.
Authors: Fryer RA, Barlett B, Galustian C, Dalgleish AG
Anticancer Res., 2011;31(11):3747-56.
Species: Human
Sample Type: Cell Lysates

Mapping the transcriptional machinery of the IL-8 gene in human bronchial epithelial cells.
Authors: Bezzerri V, Borgatti M, Finotti A, Tamanini A, Gambari R, Cabrini G
J. Immunol., 2011;187(11):6069-81.
Species: Human
Sample Type: Cell Lysates

Activation of p38 mitogen-activated protein kinase by norepinephrine in T-lineage cells.
Authors: Lajevic MD, Suleiman S, Cohen RL, Chambers DA
Immunology, 2011;132(2):197-208.
Species: Mouse
Sample Type: Cell Lysates

Anti-tumor effects of fibroblast growth factor-binding protein (FGF-BP) knockdown in colon carcinoma.
Authors: Schulze D, Plohmann P, Hobel S, Aigner A
Mol. Cancer, 2011;10(0):144.
Species: Human
Sample Type: Cell Lysates

Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.
Authors: Wei Q, Jiang H, Xiao Z
Proc. Natl. Acad. Sci. U.S.A., 2011;108(17):7004-9.
Species: Human
Sample Type: Cell Lysates

Akt cross-links IL-4 priming, stem cell factor signaling, and IgE-dependent activation in mature human mast cells.
Authors: Feuser K, Feilhauer K, Staib L, Bischoff SC, Lorentz A
Mol. Immunol., 2011;48(4):546-52.
Species: Human
Sample Type: Cell Lysates

Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
J. Exp. Med., 2010;207(4):867-80.
Species: Human
Sample Type: Cell Lysates

Somatic cell plasticity and Niemann-pick type C2 protein: adipocyte differentiation and function.
Authors: Csepeggi C, Jiang M, Frolov A
J. Biol. Chem., 2010;285(39):30347-54.
Species: Human
Sample Type: Cell Lysates

The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages.
Authors: Lane AE, Tan JT, Hawkins CL
Biochem. J., 2010;430(1):161-9.
Species: Mouse
Sample Type: Cell Lysates

The use of protein array to identify targetable receptor tyrosine kinases for treatment of human colon cancer.
Authors: Morishita A, Gong J, Nomura T
Int. J. Oncol., 2010;37(4):829-35.
Species: Human
Sample Type: Cell Lysate

NFAT and CREB regulate Kaposi's sarcoma-associated herpesvirus-induced cyclooxygenase 2 (COX-2).
Authors: Sharma-Walia N, George Paul A, Patel K
J. Virol., 2010;84(24):12733-53.
Species: Human
Sample Type: Cell Lysates

Normalization of wound healing and diabetic markers in organ cultured human diabetic corneas by adenoviral delivery of c-Met gene.
Authors: Saghizadeh M, Kramerov AA, Yu FS, Castro MG, Ljubimov AV
Invest. Ophthalmol. Vis. Sci., 2010;51(4):1970-80.
Species: Human
Sample Type: Cell Lysates

JNK is constitutively active in mantle cell lymphoma: cell cycle deregulation and polyploidy by JNK inhibitor SP600125.
Authors: Wang M, Atayar C, Rosati S, Bosga-Bouwer A, Kluin P, Visser L
J. Pathol., 2009;218(1):95-103.
Species: Human
Sample Type: Cell Lysates

Combination of EGFR and MEK1/2 inhibitor shows synergistic effects by suppressing EGFR/HER3-dependent AKT activation in human gastric cancer cells.
Authors: Yoon YK, Kim HP, Han SW, Hur HS, Oh do Y, Im SA, Bang YJ, Kim TY
Mol. Cancer Ther., 2009;8(9):2526-36.
Species: Human
Sample Type: Cell Lysates

Episodic Src activation in uveal melanoma revealed by kinase activity profiling.
Authors: Maat W, el Filali M, Dirks-Mulder A, Luyten GP, Gruis NA, Desjardins L, Boender P, Jager MJ, van der Velden PA
Br. J. Cancer, 2009;101(2):312-9.
Species: Human
Sample Type: Cell Lysates

PTEN contributes to profound PI3K/Akt signaling pathway deregulation in dystrophin-deficient dog muscle.
Authors: Feron M, Guevel L, Rouger K, Dubreil L, Arnaud MC, Ledevin M, Megeney LA, Cherel Y, Sakanyan V
Am. J. Pathol., 2009;174(4):1459-70.
Species: Canine
Sample Type: Tissue Homogenates

IL-1beta receptor blockade protects islets against pro-inflammatory cytokine induced necrosis and apoptosis.
Authors: Schwarznau A, Hanson MS, Sperger JM, Schram BR, Danobeitia JS, Greenwood KK, Vijayan A, Fernandez LA
J. Cell. Physiol., 2009;220(2):341-7.
Species: Rat
Sample Type: Cell Lysates

Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
Cancer Res., 2009;69(7):2996-3003.
Species: Human
Sample Type: Cell Lysates

Reprogramming of a subpopulation of human blood neutrophils by prolonged exposure to cytokines.
Authors: Chakravarti A, Rusu D, Flamand N, Borgeat P, Poubelle PE
Lab. Invest., 2009;89(10):1084-99.
Species: Human
Sample Type: Cell Lysates

The protein-tyrosine phosphatase, SRC homology-2 domain containing protein tyrosine phosphatase-2, is a crucial mediator of exogenous insulin-like growth factor signaling to human trophoblast.
Authors: Forbes K, West G, Garside R, Aplin JD, Westwood M
Endocrinology, 2009;150(10):4744-54.
Species: Human
Sample Type: Cell Lysates

A complex role for FGF-2 in self-renewal, survival, and adhesion of human embryonic stem cells.
Authors: Eiselleova L, Matulka K, Kriz V, Kunova M, Schmidtova Z, Neradil J, Tichy B, Dvorakova D, Pospisilova S, Hampl A, Dvorak P
Stem Cells, 2009;27(8):1847-57.
Species: Human
Sample Type: Cell Lysates

MicroRNA-330 acts as tumor suppressor and induces apoptosis of prostate cancer cells through E2F1-mediated suppression of Akt phosphorylation.
Authors: Lee KH, Chen YL, Yeh SD, Hsiao M, Lin JT, Goan YG, Lu PJ
Oncogene, 2009;28(38):3360-70.
Species: Human
Sample Type: Cell Lysates

Study of the proinflammatory role of human differentiated omental adipocytes.
Authors: Bassols J, Ortega FJ, Moreno-Navarrete JM, Peral B, Ricart W, Fernandez-Real JM
J. Cell. Biochem., 2009;107(6):1107-17.
Species: Human
Sample Type: Cell Lysates

Autocrine motility factor stimulates the invasiveness of malignant cells as well as up-regulation of matrix metalloproteinase-3 expression via a MAPK pathway.
Authors: Haga A, Funasaka T, Deyashiki Y, Raz A
FEBS Lett., 2008;582(0):1877-82.
Species: Human
Sample Type: Cell Lysates

Differential subcellular membrane recruitment of Src may specify its downstream signalling.
Authors: de Diesbach P, Medts T, Carpentier S, D'Auria L, Van Der Smissen P, Platek A, Mettlen M, Caplanusi A, van den Hove MF, Tyteca D, Courtoy PJ
Exp. Cell Res., 2008;314(7):1465-79.
Species: Canine
Sample Type: Cell Lysates

Thrombopoietin inhibits nerve growth factor-induced neuronal differentiation and ERK signalling.
Authors: Samoylenko A, Byts N, Rajalingam K, von Ahsen N, Rapp UR, Ehrenreich H, Siren AL
Cell. Signal., 2007;20(1):154-62.
Species: Rat
Sample Type: Cell Lysates

Early gene expression analyzed by cDNA microarray and real-time PCR in osteoblasts cultured with chitosan monomer.
Authors: Ganno T, Yamada S, Ohara N, Matsunaga T, Yanagiguchi K, Ikeda T, Ishizaki H, Hayashi Y
J Biomed Mater Res A, 2007;82(1):188-94.
Species: Human
Sample Type: Cell Lysates

Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells.
Authors: Eckstein N, Servan K, Girard L, Cai D, von Jonquieres G, Jaehde U, Kassack MU, Gazdar AF, Minna JD, Royer HD
J. Biol. Chem., 2007;283(2):739-50.
Species: Human
Sample Type: Cell Lysates

S100A11, an dual mediator for growth regulation of human keratinocytes.
Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
Mol. Biol. Cell, 2007;19(1):78-85.
Species: Human
Sample Type: Cell Lysates

The ERK mitogen-activated protein kinase pathway contributes to Ebola virus glycoprotein-induced cytotoxicity.
Authors: Zampieri CA, Fortin JF, Nolan GP, Nabel GJ
J. Virol., 2007;81(3):1230-40.
Species: Human
Sample Type: Cell Lysates

Identification of signaling pathways in macrophage exposed to Porphyromonas gingivalis or to its purified cell wall components.
Authors: Zhou Q, Amar S
J. Immunol., 2007;179(11):7777-90.
Species: Human
Sample Type: Cell Lysates

Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms.
Authors: Hudson TS, Hartle DK, Hursting SD, Nunez NP, Wang TT, Young HA, Arany P, Green JE
Cancer Res., 2007;67(17):8396-405.
Species: Human
Sample Type: Cell Lysates

LPS-induced TNF-alpha factor (LITAF)-deficient mice express reduced LPS-induced cytokine: Evidence for LITAF-dependent LPS signaling pathways.
Authors: Tang X, Metzger D, Leeman S, Amar S
Proc. Natl. Acad. Sci. U.S.A., 2006;103(37):13777-82.
Species: Human
Sample Type: Cell Lysates

背景

The Human Phospho-Mitogen-activated Protein Kinase (MAPK) Antibody Array (Catalog # ARY002) is an economical tool used to simultaneously detect the relative phosphorylation of 19 kinases without performing numerous immunoprecipitation and/or Western blots. Kinases are captured by 23 different antibodies spotted in duplicate on a nitrocellulose membrane. Levels of phosphorylated protein are then assessed using phospho-specific antibodies and chemiluminescence detection.

参考文献
  • Human Phospho-MAPK Array Kit
  • Intracellular Kinases
  • Positive Regulators of the Jak/STAT Pathway
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¥6312.4

货号:ARY002B

规格: 1Kit
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请您下单前确定实验服务合同结束后,样本处置情况。我们将严格按照合同约定执行。若您选择实验结束后样本,不保留,丢弃,我们会在实验结束后按国家标准自行处置掉相关实验材料。若您需要回寄相关样本,在您支付一定的运输费用后,我们将剩余样本在规定时间内返还给您。若您选择到付,需要保证您的样本是常温和蓝冰运输的,更高要求的运输条件不支持货到付款。若您不确定是否回寄,我们将保持您的样本1个月的时间,如超期,样本我们将自行处置,不在另行通知您。 如果您选择我们回寄样本给您,我们将快递信息邮件告知您,由您跟踪物流信息,并及时确认。如因您个人原因或者第三方物流原因造成的样本邮寄过程中的损耗,我们不承担任何责任。

5.实验服务种实验服务数据:

1) 我们承诺按照《实验样本信息及要求》中的实验方案进行实验并提供客观实验结果,但由于科研实验中存在诸多不确定因素以及未知难度和风险而导致结果的不确定性,不能保证所有的实验进度和实验结果完全达到甲方预期。在排除实验操作问题导致不良结果的前提下,您应尊重客观事实,承担实验结果与预期不一致的结果,包括实验结果是阴性的风险。因此在得不到预期结果的情况下,您仍应支付本次实验服务的全部已发生费用。

2) 实验服务是由专业技术达标的实验人员,遵循科学实验方法和标准操作流程,依照服务合同完成,优宁维保证将客观真实科学严谨的实验结果给到客户,但单次实验不足以阐明某些复杂的科学问题。

3) 您在收到实验数据后,5个工作日内(荧光相关实验3个工作日内)对实验数据质量提质疑,我们有义务解答。若是我们实验过程中的问题,我们承担全部责任;若是您验设计问题,您承担全部责任。我们承诺1个月的数据复核期,欢迎后续咨询。

6.客户知识产权保密协议:

1) 实验服务过程中产生的实验数据的知识产权归您所有,我们承诺不得以任何形式向除甲方以外的第三方提供该实验数据。对于相关的实验技术所有权归我们所有,您不得主张权利。

2) 我们双方共同致力于维护对方的商业机密和技术机密,包括本次合同所签订的文件,您都有义务进行保密。

7.服务承诺

如由于实验客观性需要重复实验、自然灾害、政府行为或其他乙方无法预见、无法避免和无法克服等不可抗力的客观原因造成合同无法按期履行的,我们将及时通知甲方,双方将视情况决定是否继续履行合同。如果由于客观原因造成某些实验项目不能执行的,合同结束时,退回相应款项。

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